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Heterologous Gene Expression in E.coli - Humana Press
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Heterologous Gene Expression in E.coli - encadernada, livro de bolso

2010, ISBN: 9781617379666

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvemen… mais…

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Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - Evans, Thomas C., Jr. (Editor)
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Evans, Thomas C., Jr. (Editor):

Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - encadernada, livro de bolso

2010, ISBN: 1617379662

[EAN: 9781617379666], New book, [PU: Springer Verlag], 310 pages. 10.00x7.00x1.00 inches. In Stock., Books

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Evans, Thomas C., Jr. (Editor):
Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - encadernada, livro de bolso

2010

ISBN: 9781617379666

Springer Verlag, 2010. Hardcover. New. 310 pages. 10.00x7.00x1.00 inches., Springer Verlag, 2010, 6

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Heterologous Gene Expression in E.coli : Methods and Protocols - encadernada, livro de bolso

2010, ISBN: 1617379662

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Heterologous Gene Expression in E.coli - Jr. Evans, Thomas C.; Ming-Qun Xu
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Jr. Evans, Thomas C.; Ming-Qun Xu:
Heterologous Gene Expression in E.coli - encadernada, livro de bolso

2010, ISBN: 9781617379666

Methods and Protocols, Buch, Hardcover, 2011, [PU: Humana Press Inc.], Humana Press Inc., 2010

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Heterologous Gene Expression in E.coli

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology(TM) format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

Dados detalhados do livro - Heterologous Gene Expression in E.coli


EAN (ISBN-13): 9781617379666
ISBN (ISBN-10): 1617379662
Livro de capa dura
Ano de publicação: 2011
Editor/Editora: Humana Press Inc.
307 Páginas
Peso: 0,758 kg
Língua: eng/Englisch

Livro na base de dados desde 2007-10-23T19:28:59-02:00 (Sao Paulo)
Página de detalhes modificada pela última vez em 2023-07-15T10:04:15-03:00 (Sao Paulo)
Número ISBN/EAN: 1617379662

Número ISBN - Ortografia alternativa:
1-61737-966-2, 978-1-61737-966-6
Ortografia alternativa e termos de pesquisa relacionados:
Autor do livro: ming, thomas christopher, thomas evans, qun, chris thomas
Título do livro: expression, ming, qun


Dados da editora

Autor: Thomas C. Evans, Jr.; Ming-Qun Xu
Título: Methods in Molecular Biology; Heterologous Gene Expression in E.coli - Methods and Protocols
Editora: Humana; Humana Press
310 Páginas
Ano de publicação: 2010-12-02
Totowa; NJ; US
Impresso / Feito em
Peso: 0,809 kg
Língua: Inglês
160,49 € (DE)
164,99 € (AT)
177,00 CHF (CH)
POD
XI, 310 p.

BB; Human Genetics; Hardcover, Softcover / Medizin/Klinische Fächer; Genetik, Medizin; Verstehen; Amino acid residues or moieties; Binding partners; Escherichia coli; Expression hosts; Membrane proteins; Post-translational modifications; Protein expression; Recombinant DNA; Recombinant proteins; Proteomics; Proteomics; Medical Genetics; Protein Biochemistry; Biochemie; EA; BC

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

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