Advanced Protocols in Oxidative Stress I - nuovo livro

EAN (ISBN-13): 9781617378812
ISBN (ISBN-10): 161737881X
Livro de capa dura
Livro de bolso
Ano de publicação: 2011
Editor/Editora: Humana Press Inc.
444 Páginas
Língua: eng/Englisch

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Página de detalhes modificada pela última vez em 2021-08-23T13:49:43-03:00 (Sao Paulo)
Número ISBN/EAN: 161737881X

Número ISBN - Ortografia alternativa:
1-61737-881-X, 978-1-61737-881-2
Ortografia alternativa e termos de pesquisa relacionados:
Autor do livro: armstrong
Título do livro: advanced molecular biology

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Dados da editora

Autor: Donald Armstrong
Título: Methods in Molecular Biology; Advanced Protocols in Oxidative Stress I
Editora: Humana; Humana Press
430 Páginas
Ano de publicação: 2011-01-20
Totowa; NJ; US
Impresso / Feito em
Língua: Inglês
155,14 € (DE)
159,49 € (AT)
171,50 CHF (CH)
POD
XIV, 430 p. 100 illus., 1 illus. in color.

BC; Hardcover, Softcover / Biologie/Biochemie, Biophysik; Biochemie; Verstehen; Antioxidant supplementation; Biosystems technology; Free radical reactions; Microarray; Oxidative stress reactions; Termination; gene expression; gene transcription; genes; membrane potential; molecular biology; transcription; Biochemistry; Cell Biology; Cellular Stress; Zellbiologie (Zytologie); BB

Reactive Oxygen and Nitrogen Techniques.- Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cells, and Tissues.- A Rapid and Selective Mass Spectrometric Method for the Identification of Nitrated Proteins.- An Easy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting.- Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization in Studies of Oxidative and Nitrosative Stress.- Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL.- High Performance Liquid Chromatography/Electron Spin Resonance/Mass Spectrometry Analyses of Lipid-Derived Radicals.- EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/•OOH and Immunospin-Trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer System.- Determination of High Mitochondrial Membrane Potential in Spermatozoa Loaded with the Mitochondrial Probe 5,5?,6,6?-Tetrachloro-1,1?,3,3?-Tetraethylbenzimidazolyl-Carbocyanine Iodide (JC-1) by Using Fluorescence-Activated Flow Cytometry.- 2,2,6,6-Tetramethylpiperidin-1-Oxyl Probes for Evaluating Oxidative Stress on the Cell Membrane and Mitochondria.- Zymographical Techniques for Detectionof Matrix Metalloproteinases.- The Use of In Vivo Microdialysis Techniques to Detect Extracellular ROS in Resting and Contracting Skeletal Muscle.- Cell-Free Antibody Capture Method for Analysis of Detergent-Resistant Membrane Rafts.- Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids.- Antioxidant Technology and Application.- Cupric Ion Reducing Antioxidant Capacity Assay for Food Antioxidants: Vitamins, Polyphenolics, and Flavonoids in Food Extracts.- Redox Property of Ribonucleotide ReductaseSmall Subunit M2 andp53R2.- Antioxidant QSAR Modeling as Exemplified on Polyphenols.- Immunohistochemical Staining of Cyclooxygenases with Monoclonal Antibodies.- Examining the Endogenous Antioxidant Response Through Immunofluorescent Analysis of Nrf2 in Tissue.- Determination of Oxidized and Reduced CoQ10 and CoQ9 in Human Plasma/Serum Using HPLC-ECD.- Paraoxonases (PON1, PON2, PON3) Analyses In Vitro and In Vivo in Relation to Cardiovascular Diseases.- Preparation, Characterization, and Use of Antioxidant-Liposomes.- Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus stearothermophilus.- Biolistic Mediated Production of Transgenic Oil Palm.- Preparation of a Multi-antioxidant Formulation.- Gene Expression.- A Functional Genomics Approach to Identify and Characterize Oxidation Resistance Genes.- Genome-Wide Overexpression Screen for Activators of Antioxidant Gene Transcription.- Method for Conducting Microarray Study of Oxidative Stress Induced Gene Expression.- A Systems Approach Demonstrating Sphingolipid-Dependent Transcription in Stress Responses.- Bioluminescence: Imaging Modality for In Vitro and In Vivo Gene Expression.- Stem Cells.- Reactive Oxygen Species and Upregulation of NADPH Oxidases in Mechanotransduction of Embryonic Stem Cells.- Biostatistics.- Pooling Data When Analyzing Biomarkers Subject to a Limit of Detection.
Describes novel probes for detecting ROS and RNS Provides theoretical models to predict structural features of polyphenol antioxidants Provides automated assays for ROS metabolites and antioxidant capacity Use of HPLC-nanoHPLC-ESR-MS-ESI-ECD and high-voltage capillary electrophoresis Shows how correlative transmission microscopy allows for in-situ visualization of localized sites of oxidative stress Illustrates immunohistochemistry and bioluminescence techniques Detecting extracellular ROS by microdialysis Describes microarray technology Production of antibodies to detergent-resistant lipid rafts Stress response and gene expression Discusses antioxidant modeling, liposome preparation , response element activity and preparation of multi-antioxidant pharmaceutical formulations Describes HDL-associated paraoxonase enzyme activity in knock-out and transgenic mice